Lysostaphin: a New Bacteriolytic Agent for the Staphylococcus.
نویسندگان
چکیده
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منابع مشابه
Cloning, Expression, and Purification of Recombinant Lysostaphin From Staphylococcus simulans
BACKGROUND Staphylococcus aureus is one of the most common causes of nosocomial infections and its resistance to antibiotics is a global concern. Lysostaphin is an antimicrobial agent belonging to a major class of antimicrobial peptides and proteins known as the bacteriocins. It exhibits a high degree of anti-staphylococcal bacteriolytic activity. OBJECTIVES In this study, high level of recom...
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Lysostaphin is a zinc-metalloenzyme which has a molecular weight of 25,000 when determined by sedimentation equilibrium in dilute aqueous salt solutions. Combination of the sedimentation coefficient, sZO,lo = 2.32, and diffusion coefficient, DBo,w = 7.83, gave a native molecular weight of 25,800. Sedimentation equilibrium of the protein in 6 M guanidine hydrochloride, with and without 2-mercapt...
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Despite intense efforts by the medical and pharmaceutical communities, Staphylococcus aureus continues to be a pervasive pathogen that causes a myriad of diseases and a high level of morbidity and mortality among infected patients. Thus, discovering or designing novel therapeutics able to kill both drug-resistant and drug-sensitive S. aureus remains a top priority. Bacteriolytic enzymes, mostly...
متن کاملStructure-based redesign of lysostaphin yields potent antistaphylococcal enzymes that evade immune cell surveillance
Staphylococcus aureus infections exert a tremendous burden on the health-care system, and the threat of drug-resistant strains continues to grow. The bacteriolytic enzyme lysostaphin is a potent antistaphylococcal agent with proven efficacy against both drug-sensitive and drug-resistant strains; however, the enzyme's own bacterial origins cause undesirable immunogenicity and pose a barrier to c...
متن کاملکلونینگ، بیان، خالص سازی و ارزیابی آنزیم لیزواستافین نوترکیب با استفاده از سیستم کلونینگ pBAD
Background and purpose: Staphylococcus aureus is an important nosocomial pathogen which causes some diseases such as endocarditis, osteomyelitis, pneumonia, toxic shock syndrome, and food poisoning. The excessive and inappropriate use of antibiotics in the treatment of these diseases causes resistance to many antibiotics. Lysostaphin is an effective agent in the treatment of staphylococcal infe...
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عنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 51 شماره
صفحات -
تاریخ انتشار 1964